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The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. <t>(F)</t> <t>Live/Dead</t> staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).
Dead Cell Double Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Biocompatibility and cell migration of the composite stent. <t>(A)</t> <t>Live/dead</t> staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.
Dead Cell Staining Kit, supplied by Keygen Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Miltenyi Biotec macs dead cell removal kit
Biocompatibility and cell migration of the composite stent. <t>(A)</t> <t>Live/dead</t> staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.
Macs Dead Cell Removal Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

Journal: Bioactive Materials

Article Title: Microenvironment-responsive injectable dynamic hydrogel for sequential antioxidant and tissue regeneration therapy of radiation-induced skin injury

doi: 10.1016/j.bioactmat.2026.03.057

Figure Lengend Snippet: The antibacterial activity and biocompatibility of hydrogels. (A) Antibacterial image of hydrogels against MRSA . (B) Antibacterial image of hydrogels against E.coil . (C, D) Relative bacterial viability of MRSA and E.coil after 24 h of co-incubation with the hydrogels. (E)Representative crystal violet-stained images of MRSA and E. coli biofilms after different treatments. (F) Live/Dead staining of L929 cells after co-culture with different hydrogel groups. (G) Hemocompatibility test of different hydrogel groups. (H) CCK8 of L929 cells after co-culture with different hydrogel groups. (*P < 0.05, **P < 0.01, ***P < 0.001, mean ± SD, n = 3).

Article Snippet: The Live/Dead Cell Double Staining Kit was supplied by Jiangsu KeyGEN BioTECH Co., Ltd., and the Cell Counting Kit-8 (CCK-8) was obtained from Beijing Solarbio Science & Technology Co., Ltd.

Techniques: Activity Assay, Incubation, Staining, Co-Culture Assay

Biocompatibility and cell migration of the composite stent. (A) Live/dead staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.

Journal: Bioactive Materials

Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula

doi: 10.1016/j.bioactmat.2026.01.014

Figure Lengend Snippet: Biocompatibility and cell migration of the composite stent. (A) Live/dead staining of IEC-6 cells cultured with the stent, showing cell viability. (B) Hemocompatibility of the stent: (i) hemolysis assay of red blood cells, (ii) corresponding hemolysis percentage. (C) Representative images of cell migration in the scratch assay. (D) Quantification of (i) migration area and (ii) percentage closure over time. (E) Representative Transwell images of migrated cells. (F) Quantification of migrated cells in the Transwell assay. Data were presented as mean ± SD (n = 3). ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. Gel represents the G 2 S 4 hydrogel group, and Comp represents the composite stent group; the same abbreviations are used in the following figures.

Article Snippet: Live/Dead Cell Staining Kit was bought from KeyGEN BioTech (Jiangsu, China).

Techniques: Migration, Staining, Cell Culture, Hemolysis Assay, Wound Healing Assay, Transwell Assay